Abstract

Author:
Jialei Xiong, Xiaochen Yue, Guanyan Li, Wanxi Peng

Doi: 10.26480/jhcdc.02.2025.43.49

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

This study analyzes Arctium lappa L. (burdock) ethanol extract against NCI-H292 lung cancer cells. Comprehensive profiling identified bioactive components: Gas Chromatography-Mass Spectrometry detected volatile compounds including α-linolenic acid and Phytol; Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry revealed non-volatile constituents such as Asarinin B, Embelin, Tanshinone IIA, Protocatechuic aldehyde, and Bergenin. Summer root extract demonstrated superior cytotoxic activity with a 40.61% killing rate via MTT assay; correlated Fourier transform infrared spectroscopy spectral signatures suggested unique compositional features. Network pharmacology predicted 148 potential targets, with protein-protein interaction analysis prioritizing key nodes AKT1, EGFR, and TNF. Pathway enrichment implicated MAPK, NF kappa B, and small cell lung cancer signaling cascades. Molecular docking confirmed strong binding affinities between active constituents and therapeutic targets. These integrated findings establish that summer-harvested burdock root exerts multi-component, multi-target anti-lung cancer activity, functioning through synergistic modulation of critical targets including AKT1, EGFR, and TNF across multiple pathways.